Quantifying mitochondrial DNA copy number using robust regression to interpret real time PCR results

BACKGROUND: Real time PCR (rtPCR) is a quantitative assay to determine the relative DNA copy number in a sample versus a reference. The [Formula: see text] method is the standard for the analysis of the output data generated by an rtPCR experiment. We developed an alternative based on fitting a robu...

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Détails bibliographiques
Publié dans:BMC Res Notes
Auteurs principaux: Refinetti, Paulo, Warren, David, Morgenthaler, Stephan, Ekstrøm, Per O.
Format: Artigo
Langue:Inglês
Publié: BioMed Central 2017
Sujets:
Technical Note
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5683470/
https://ncbi.nlm.nih.gov/pubmed/29132417
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13104-017-2913-1
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