Periplasmic form of dipeptidyl aminopeptidase IV from Pseudoxanthomonas mexicana WO24: purification, kinetic characterization, crystallization and X-ray crystallographic analysis

Dipeptidyl aminopeptidase IV (DAP IV or DPP IV) from Pseudoxanthomonas mexicana WO24 (PmDAP IV) preferentially cleaves substrate peptides with Pro or Ala at the P1 position [NH(2)-P2-P1(Pro/Ala)-P1′-P2′…]. For crystallo­graphic studies, the periplasmic form of PmDAP IV was overproduced in Escherichi...

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Detalhes bibliográficos
Publicado no:Acta Crystallogr F Struct Biol Commun
Main Authors: Roppongi, Saori, Tateoka, Chika, Fujimoto, Mayu, Iizuka, Ippei, Morisawa, Saori, Nakamura, Akihiro, Honma, Nobuyuki, Suzuki, Yoshiyuki, Shida, Yosuke, Ogasawara, Wataru, Tanaka, Nobutada, Sakamoto, Yasumitsu, Nonaka, Takamasa
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2017
Assuntos:
Research Communications
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5683029/
https://ncbi.nlm.nih.gov/pubmed/29095153
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X17014911
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