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Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis
The GFP-based superecliptic pHluorin (SEP) enables detection of exocytosis and endocytosis, but its performance has not been duplicated in red fluorescent protein scaffolds. Here we describe “semisynthetic” pH-sensitive protein conjugates with organic fluorophores, carbofluorescein, and Virginia Ora...
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| Publicado no: | Nat Commun |
|---|---|
| Main Authors: | , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group UK
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5680258/ https://ncbi.nlm.nih.gov/pubmed/29123102 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-017-01752-5 |
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