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Protein Expression and Purification of the Hsp90-Cdc37-Cdk4 Kinase Complex from Saccharomyces cerevisiae

Interactions between Hsp90, its co-chaperone Cdc37 and kinases have been biochemically studied for over three decades and have been shown to be functionally important in organisms from yeast to humans. However, formation of a stable complex for structural studies has been elusive. In this protocol w...

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Detalhes bibliográficos
Publicado no:Bio Protoc
Main Authors: Verba, Kliment A., Agard, David A.
Formato: Artigo
Idioma:Inglês
Publicado em: Bio-Protocol 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5669387/
https://ncbi.nlm.nih.gov/pubmed/29104897
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2563
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