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Intracellular Cytokine Staining on PBMCs Using CyTOF™ Mass Cytometry
In this protocol, we use a CyTOF™ mass cytometry to collect single-cell data on a large number of cytokines/chemokines as well as cell-surface proteins that characterize T cells and other immune cells. The current selected mass window in AW 103–203 includes the lanthanides used for most antibody lab...
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| Veröffentlicht in: | Bio Protoc |
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| Hauptverfasser: | , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2015
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5669382/ https://ncbi.nlm.nih.gov/pubmed/29104886 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.1370 |
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