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Intracellular Cytokine Staining on PBMCs Using CyTOF™ Mass Cytometry

In this protocol, we use a CyTOF™ mass cytometry to collect single-cell data on a large number of cytokines/chemokines as well as cell-surface proteins that characterize T cells and other immune cells. The current selected mass window in AW 103–203 includes the lanthanides used for most antibody lab...

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Publicat a:Bio Protoc
Autors principals: Lin, Dongxia, Gupta, Sheena, Maecker, Holden T.
Format: Artigo
Idioma:Inglês
Publicat: 2015
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5669382/
https://ncbi.nlm.nih.gov/pubmed/29104886
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.1370
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