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CRISPRi is not strand-specific at all loci and redefines the transcriptional landscape
CRISPRi, an adapted CRISPR-Cas9 system, is proposed to act as a strand-specific roadblock to repress transcription in eukaryotic cells using guide RNAs (sgRNAs) to target catalytically inactive Cas9 (dCas9) and offers an alternative to genetic interventions for studying pervasive antisense transcrip...
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| Publicado no: | eLife |
|---|---|
| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
eLife Sciences Publications, Ltd
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5665645/ https://ncbi.nlm.nih.gov/pubmed/29058669 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.29878 |
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