TA-GC cloning: A new simple and versatile technique for the directional cloning of PCR products for recombinant protein expression

During the last few decades, the recombinant protein expression finds more and more applications. The cloning of protein-coding genes into expression vectors is required to be directional for proper expression, and versatile in order to facilitate gene insertion in multiple different vectors for exp...

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Détails bibliographiques
Publié dans:PLoS One
Auteurs principaux: Niarchos, Athanasios, Siora, Anastasia, Konstantinou, Evangelia, Kalampoki, Vasiliki, Lagoumintzis, George, Poulas, Konstantinos
Format: Artigo
Langue:Inglês
Publié: Public Library of Science 2017
Sujets:
Research Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5665511/
https://ncbi.nlm.nih.gov/pubmed/29091919
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0186568
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