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A universal cloning method based on yeast homologous recombination that is simple, efficient, and versatile

Cloning by homologous recombination (HR) in Saccharomyces cerevisiae is an extremely efficient and cost-effective alternative to other methods of recombinant DNA technologies. Unfortunately, it is incompatible with all the various specialized plasmids currently used in microbiology and biomedical re...

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Bibliografske podrobnosti
izdano v:J Microbiol Methods
Main Authors: Joska, Tammy M., Mashruwala, Ameya, Boyd, Jeffrey M., Belden, William J.
Format: Artigo
Jezik:Inglês
Izdano: 2014
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4521215/
https://ncbi.nlm.nih.gov/pubmed/24418681
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mimet.2013.11.013
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