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Psychrophilic proteases dramatically reduce single-cell RNA-seq artifacts: a molecular atlas of kidney development

Single-cell RNA-seq is a powerful technique. Nevertheless, there are important limitations, including the technical challenges of breaking down an organ or tissue into a single-cell suspension. Invariably, this has required enzymatic incubation at 37°C, which can be expected to result in artifactual...

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Dettagli Bibliografici
Pubblicato in:Development
Autori principali: Adam, Mike, Potter, Andrew S., Potter, S. Steven
Natura: Artigo
Lingua:Inglês
Pubblicazione: The Company of Biologists Ltd 2017
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5665481/
https://ncbi.nlm.nih.gov/pubmed/28851704
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/dev.151142
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