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Psychrophilic proteases dramatically reduce single-cell RNA-seq artifacts: a molecular atlas of kidney development
Single-cell RNA-seq is a powerful technique. Nevertheless, there are important limitations, including the technical challenges of breaking down an organ or tissue into a single-cell suspension. Invariably, this has required enzymatic incubation at 37°C, which can be expected to result in artifactual...
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| Publicado no: | Development |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The Company of Biologists Ltd
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5665481/ https://ncbi.nlm.nih.gov/pubmed/28851704 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/dev.151142 |
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