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Detection and Visualization of Specific Gene Transcripts by in situ RT-PCR in Nematode-Infected Arabidopsis Root Tissue

This protocol describes an effective method of in situ RT-PCR that was developed to localize specific gene expression directly in thin cross-sections of nematode feeding sites induced by the cyst nematode Heterodera schachtii (H. schachtii) or the root-knot nematode Meloidogyne incognita (M. incogni...

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Detalhes bibliográficos
Publicado no:Bio Protoc
Autor principal: Wieczorek, Krzysztof
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5659371/
https://ncbi.nlm.nih.gov/pubmed/29082278
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.1597
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