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Detection and Visualization of Specific Gene Transcripts by in situ RT-PCR in Nematode-Infected Arabidopsis Root Tissue

This protocol describes an effective method of in situ RT-PCR that was developed to localize specific gene expression directly in thin cross-sections of nematode feeding sites induced by the cyst nematode Heterodera schachtii (H. schachtii) or the root-knot nematode Meloidogyne incognita (M. incogni...

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Vydáno v:Bio Protoc
Hlavní autor: Wieczorek, Krzysztof
Médium: Artigo
Jazyk:Inglês
Vydáno: 2015
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5659371/
https://ncbi.nlm.nih.gov/pubmed/29082278
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.1597
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