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Chemical crosslinking and mass spectrometry to elucidate the topology of integral membrane proteins
Here we made an attempt to obtain partial structural information on the topology of multispan integral membrane proteins of yeast by isolating organellar membranes, removing peripheral membrane proteins at pH 11.5 and introducing chemical crosslinks between vicinal amino acids either using homo- or...
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| Publicado no: | PLoS One |
|---|---|
| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5658093/ https://ncbi.nlm.nih.gov/pubmed/29073188 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0186840 |
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