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Overexpression and purification of Dicer and accessory proteins for biochemical and structural studies
The Dicer family of ribonucleases plays a key role in small RNA-based regulatory pathways by generating short dsRNA fragments that modulate expression of endogenous genes, or protect the host from invasive nucleic acids. Beginning with its initial discovery, biochemical characterization of Dicer has...
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| Udgivet i: | Methods |
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| Main Authors: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2017
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5658042/ https://ncbi.nlm.nih.gov/pubmed/28723582 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2017.07.012 |
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