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Quantitative assessment of the effects of trypsin digestion methods on affinity purification-mass spectrometry-based protein-protein interaction analysis

Affinity purification-mass spectrometry (AP-MS) has become the method of choice for discovering protein-protein interactions (PPIs) under native conditions. The success of AP-MS depends on the efficiency of trypsin digestion and the recovery of the tryptic peptides for MS analysis. Several different...

Täydet tiedot

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Bibliografiset tiedot
Julkaisussa:J Proteome Res
Päätekijät: Zhang, Yueqing, Sun, Hong, Zhang, Jing, Brasier, Allan R., Zhao, Yingxin
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: 2017
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC5656008/
https://ncbi.nlm.nih.gov/pubmed/28726418
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.jproteome.7b00432
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