Improving the thermal stability of cellobiohydrolase Cel7A from Hypocrea jecorina by directed evolution

Secreted mixtures of Hypocrea jecorina cellulases are able to efficiently degrade cellulosic biomass to fermentable sugars at large, commercially relevant scales. H. jecorina Cel7A, cellobiohydrolase I, from glycoside hydrolase family 7, is the workhorse enzyme of the process. However, the thermal s...

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Bibliographische Detailangaben
Veröffentlicht in:J Biol Chem
Hauptverfasser: Goedegebuur, Frits, Dankmeyer, Lydia, Gualfetti, Peter, Karkehabadi, Saeid, Hansson, Henrik, Jana, Suvamay, Huynh, Vicky, Kelemen, Bradley R., Kruithof, Paulien, Larenas, Edmund A., Teunissen, Pauline J. M., Ståhlberg, Jerry, Payne, Christina M., Mitchinson, Colin, Sandgren, Mats
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Biochemistry and Molecular Biology 2017
Schlagworte:
Enzymology
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5655518/
https://ncbi.nlm.nih.gov/pubmed/28860192
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M117.803270
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