Formation of the chaperonin complex studied by 2D NMR spectroscopy

We studied the interaction between GroES and a single-ring mutant (SR1) of GroEL by the NMR titration of (15)N-labeled GroES with SR1 at three different temperatures (20, 25 and 30°C) in the presence of 3 mM ADP in 100 mM KCl and 10 mM MgCl(2) at pH 7.5. We used SR1 instead of wild-type double-ring...

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Veröffentlicht in:PLoS One
Hauptverfasser: Takenaka, Toshio, Nakamura, Takashi, Yanaka, Saeko, Yagi-Utsumi, Maho, Chandak, Mahesh S., Takahashi, Kazunobu, Paul, Subhankar, Makabe, Koki, Arai, Munehito, Kato, Koichi, Kuwajima, Kunihiro
Format: Artigo
Sprache:Inglês
Veröffentlicht: Public Library of Science 2017
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Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5653362/
https://ncbi.nlm.nih.gov/pubmed/29059240
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0187022
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