The use of spin desalting columns in DMSO-quenched H/D-exchange NMR experiments

Dimethylsulfoxide (DMSO)-quenched hydrogen/deuterium (H/D)-exchange is a powerful method to characterize the H/D-exchange behaviors of proteins and protein assemblies, and it is potentially useful for investigating non-protected fast-exchanging amide protons in the unfolded state. However, the metho...

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Detalhes bibliográficos
Main Authors: Chandak, Mahesh S, Nakamura, Takashi, Takenaka, Toshio, Chaudhuri, Tapan K, Yagi-Utsumi, Maho, Chen, Jin, Kato, Koichi, Kuwajima, Kunihiro
Formato: Artigo
Idioma:Inglês
Publicado em: Wiley Subscription Services, Inc., A Wiley Company 2013
Assuntos:
Methods and Applications
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3610054/
https://ncbi.nlm.nih.gov/pubmed/23339068
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.2221
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