Multicolor Stimulated Emission Depletion (STED) Microscopy to Generate High-resolution Images of Respiratory Syncytial Virus Particles and Infected Cells

Human respiratory syncytial virus (RSV) infection in human lung epithelial A549 cells induces filopodia, cellular protrusions consisting of F-actin, that extend to neighboring uninfected cells ( Mehedi et al., 2016 ). High-resolution imaging via stimulated emission depletion (STED) microscopy reveal...

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Detalles Bibliográficos
Publicado en:Bio Protoc
Autores principales: Mehedi, Masfique, Smelkinson, Margery, Kabat, Juraj, Ganesan, Sundar, Collins, Peter L., Buchholz, Ursula J.
Formato: Artigo
Lenguaje:Inglês
Publicado: Bio-Protocol 2017
Materias:
Methods Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC5650235/
https://ncbi.nlm.nih.gov/pubmed/29057295
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2543
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