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STED-SPIM: Stimulated Emission Depletion Improves Sheet Illumination Microscopy Resolution

We demonstrate the first, to our knowledge, integration of stimulated emission depletion (STED) with selective plane illumination microscopy (SPIM). Using this method, we were able to obtain up to 60% improvements in axial resolution with lateral resolution enhancements in control samples and zebraf...

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Bibliographic Details
Main Authors: Friedrich, Mike, Gan, Qiang, Ermolayev, Vladimir, Harms, Gregory S.
Format: Artigo
Language:Inglês
Published: The Biophysical Society 2011
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3077687/
https://ncbi.nlm.nih.gov/pubmed/21504720
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2010.12.3748
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