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Performance and consistency of a fluorescence-based high-throughput screening assay for use in Babesia drug screening in mice

In this study, we evaluated the validity of a fluorescence-based assay using SYBR Green I (SG I) stain for screening antibabesial compounds against B. microti in mice. Two different hematocrits (HCTs; 2.5% and 5%) were used. Correlating relative fluorescence units (RFUs) with parasitemia showed sign...

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Vydáno v:Sci Rep
Hlavní autoři: Rizk, Mohamed Abdo, El-Sayed, Shimaa Abd El-Salam, AbouLaila, Mahmoud, Eltaysh, Rasha, Yokoyama, Naoaki, Igarashi, Ikuo
Médium: Artigo
Jazyk:Inglês
Vydáno: Nature Publishing Group UK 2017
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5643553/
https://ncbi.nlm.nih.gov/pubmed/29038534
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-13052-5
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