Isothermal amplification of environmental DNA (eDNA) for direct field-based monitoring and laboratory confirmation of Dreissena sp.

Loop-mediated isothermal amplification (LAMP) of aquatic invasive species environmental DNA (AIS eDNA) was used for rapid, sensitive, and specific detection of Dreissena sp. relevant to the Great Lakes (USA) basin. The method was validated for two uses including i) direct amplification of eDNA using...

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Опубликовано в: :PLoS One
Главные авторы: Williams, Maggie R., Stedtfeld, Robert D., Engle, Cathrine, Salach, Paul, Fakher, Umama, Stedtfeld, Tiffany, Dreelin, Erin, Stevenson, R. Jan, Latimore, Jo, Hashsham, Syed A.
Формат: Artigo
Язык:Inglês
Опубликовано: Public Library of Science 2017
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Research Article
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC5643059/
https://ncbi.nlm.nih.gov/pubmed/29036210
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0186462
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