Isothermal amplification of environmental DNA (eDNA) for direct field-based monitoring and laboratory confirmation of Dreissena sp.

Loop-mediated isothermal amplification (LAMP) of aquatic invasive species environmental DNA (AIS eDNA) was used for rapid, sensitive, and specific detection of Dreissena sp. relevant to the Great Lakes (USA) basin. The method was validated for two uses including i) direct amplification of eDNA using...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Williams, Maggie R., Stedtfeld, Robert D., Engle, Cathrine, Salach, Paul, Fakher, Umama, Stedtfeld, Tiffany, Dreelin, Erin, Stevenson, R. Jan, Latimore, Jo, Hashsham, Syed A.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2017
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5643059/
https://ncbi.nlm.nih.gov/pubmed/29036210
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0186462
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