Using transmission electron microscopy and 3View® to determine collagen fibril size and three-dimensional organization

Collagen fibrils are the major tensile element in vertebrate tissues where they occur as ordered bundles in the extracellular matrix. Abnormal fibril assembly and organization results in scarring, fibrosis, poor wound healing and connective tissue diseases. Transmission electron microscopy (TEM) is...

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Veröffentlicht in:Nat Protoc
Hauptverfasser: Starborg, Tobias, Kalson, Nicholas S., Lu, Yinhui, Mironov, Aleksandr, Cootes, Timothy F., Holmes, David F., Kadler, Karl E.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2013
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5642902/
https://ncbi.nlm.nih.gov/pubmed/23807286
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2013.086
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