Single-Step qPCR and dPCR Detection of Diverse CRISPR-Cas9 Gene Editing Events in Vivo

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR-associated protein 9 (Cas9)-based technology is currently the most flexible means to create targeted mutations by recombination or indel mutations by nonhomologous end joining. During mouse transgenesis, recombinant and indel...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:G3 (Bethesda)
Main Authors: Falabella, Micol, Sun, Linqing, Barr, Justin, Pena, Andressa Z., Kershaw, Erin E., Gingras, Sebastien, Goncharova, Elena A., Kaufman, Brett A.
Format: Artigo
Jezik:Inglês
Izdano: Genetics Society of America 2017
Teme:
Investigations
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5633400/
https://ncbi.nlm.nih.gov/pubmed/28860183
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/g3.117.300123
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