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Droplet digital PCR for rapid enumeration of viral genomes and particles from cells and animals infected with orthopoxviruses

Droplet digital polymerase chain reaction (ddPCR) was adapted for quantifying the number of orthopoxviral genomes in purified virus samples, infected cell lysates and tissues of infected animals. In contrast to the more commonly used qPCR, the newer ddPCR provides absolute numbers of DNA copies in s...

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Detalhes bibliográficos
Publicado no:Virology
Main Authors: Americo, Jeffrey L., Earl, Patricia L., Moss, Bernard
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5623639/
https://ncbi.nlm.nih.gov/pubmed/28802157
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.virol.2017.08.005
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