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Expression of short hairpin RNAs using the compact architecture of retroviral microRNA genes

Short hairpin RNAs (shRNAs) are effective in generating stable repression of gene expression. RNA polymerase III (RNAP III) type III promoters (U6 or H1) are typically used to drive shRNA expression. While useful for some knockdown applications, the robust expression of U6/H1-driven shRNAs can induc...

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Detalles Bibliográficos
Publicado en:Nucleic Acids Res
Main Authors: Burke, James M., Kincaid, Rodney P., Aloisio, Francesca, Welch, Nicole, Sullivan, Christopher S.
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 2017
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5622367/
https://ncbi.nlm.nih.gov/pubmed/28973449
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkx653
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