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Expression of short hairpin RNAs using the compact architecture of retroviral microRNA genes
Short hairpin RNAs (shRNAs) are effective in generating stable repression of gene expression. RNA polymerase III (RNAP III) type III promoters (U6 or H1) are typically used to drive shRNA expression. While useful for some knockdown applications, the robust expression of U6/H1-driven shRNAs can induc...
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| Udgivet i: | Nucleic Acids Res |
|---|---|
| Main Authors: | , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
2017
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5622367/ https://ncbi.nlm.nih.gov/pubmed/28973449 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkx653 |
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