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Labelling HaloTag Fusion Proteins with HaloTag Ligand in Living Cells
HaloTag has been widely used to label proteins in vitro and in vivo ( Los et al., 2008 ). In this protocol, we describe labelling HaloTag-Cbx fusion proteins by HaloTag ligands for live-cell single-molecule imaging ( Zhen et al., 2016 ).
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| Izdano u: | Bio Protoc |
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| Glavni autori: | , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Bio-Protocol
2017
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5621771/ https://ncbi.nlm.nih.gov/pubmed/28966948 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2526 |
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