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Producing GST-Cbx7 Fusion Proteins from Escherichia coli

This protocol describes the production of GST-Cbx7 fusion proteins from E. coli, originally developed in the recent publication ( Zhen et al., 2016 ). The pGEX-6P-1-GST plasmids encoding the Cbx7 variants were transformed into BL21 competent cells. The fusion protein production was induced by isopro...

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Detalhes bibliográficos
Publicado no:Bio Protoc
Main Authors: Huynh, Thao Ngoc, Ren, Xiaojun
Formato: Artigo
Idioma:Inglês
Publicado em: Bio-Protocol 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5621756/
https://ncbi.nlm.nih.gov/pubmed/28966944
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2333
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