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Simple Elimination of Background Fluorescence in Formalin-Fixed Human Brain Tissue for Immunofluorescence Microscopy

Immunofluorescence is a common method used to visualize subcellular compartments and to determine the localization of specific proteins within a tissue sample. A great hindrance to the acquisition of high quality immunofluorescence images is endogenous autofluorescence of the tissue caused by aging...

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Bibliographische Detailangaben
Veröffentlicht in:J Vis Exp
Hauptverfasser: Sun, Yulong, Ip, Philbert, Chakrabartty, Avijit
Format: Artigo
Sprache:Inglês
Veröffentlicht: MyJove Corporation 2017
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5614400/
https://ncbi.nlm.nih.gov/pubmed/28892031
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56188
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