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The Separation and Quantitation of Peptides with and without Oxidation of Methionine and Deamidation of Asparagine Using Hydrophilic Interaction Liquid Chromatography with Mass Spectrometry (HILIC-MS)

Peptides with deamidated asparagine residues and oxidized methionine residues are often not resolved sufficiently to allow quantitation of their native and modified forms using reversed phase (RP) chromatography. The accurate quantitation of these modifications is vital in protein biotherapeutic ana...

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Detalhes bibliográficos
Publicado no:J Am Soc Mass Spectrom
Main Authors: Badgett, Majors J., Boyes, Barry, Orlando, Ron
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5612368/
https://ncbi.nlm.nih.gov/pubmed/28050869
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13361-016-1565-z
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