An Optimized Transient Dual Luciferase Assay for Quantifying MicroRNA Directed Repression of Targeted Sequences

Studies investigating the action of small RNAs on computationally predicted target genes require some form of experimental validation. Classical molecular methods of validating microRNA action on target genes are laborious, while approaches that tag predicted target sequences to qualitative reporter...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Front Plant Sci
Main Authors: Moyle, Richard L., Carvalhais, Lilia C., Pretorius, Lara-Simone, Nowak, Ekaterina, Subramaniam, Gayathery, Dalton-Morgan, Jessica, Schenk, Peer M.
Format: Artigo
Jezik:Inglês
Izdano: Frontiers Media S.A. 2017
Teme:
Plant Science
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5611435/
https://ncbi.nlm.nih.gov/pubmed/28979287
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fpls.2017.01631
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