An Optimized Transient Dual Luciferase Assay for Quantifying MicroRNA Directed Repression of Targeted Sequences

Studies investigating the action of small RNAs on computationally predicted target genes require some form of experimental validation. Classical molecular methods of validating microRNA action on target genes are laborious, while approaches that tag predicted target sequences to qualitative reporter...

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Detalhes bibliográficos
Publicado no:Front Plant Sci
Main Authors: Moyle, Richard L., Carvalhais, Lilia C., Pretorius, Lara-Simone, Nowak, Ekaterina, Subramaniam, Gayathery, Dalton-Morgan, Jessica, Schenk, Peer M.
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2017
Assuntos:
Plant Science
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5611435/
https://ncbi.nlm.nih.gov/pubmed/28979287
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fpls.2017.01631
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