An Optimized Transient Dual Luciferase Assay for Quantifying MicroRNA Directed Repression of Targeted Sequences

Studies investigating the action of small RNAs on computationally predicted target genes require some form of experimental validation. Classical molecular methods of validating microRNA action on target genes are laborious, while approaches that tag predicted target sequences to qualitative reporter...

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Bibliographische Detailangaben
Veröffentlicht in:Front Plant Sci
Hauptverfasser: Moyle, Richard L., Carvalhais, Lilia C., Pretorius, Lara-Simone, Nowak, Ekaterina, Subramaniam, Gayathery, Dalton-Morgan, Jessica, Schenk, Peer M.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Frontiers Media S.A. 2017
Schlagworte:
Plant Science
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5611435/
https://ncbi.nlm.nih.gov/pubmed/28979287
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fpls.2017.01631
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