A protocol for the generation of xeno-free, cGMP-compliant patient-specific iPSCs from skin biopsy

This unit describes protocols for the generation of clinical-grade patient-specific induced pluripotent stem cell (iPSC)-derived retinal cells from patients with inherited retinal degenerative blindness. Specifically, we describe how using xeno-free reagents in an ISO class 5 environment, one can is...

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Bibliografske podrobnosti
izdano v:Curr Protoc Stem Cell Biol
Main Authors: Wiley, Luke A., Anfinson, Kristin R., Cranston, Cathryn M., Kaalberg, Emily E., Collins, Malia M., Mullins, Robert F., Stone, Edwin M., Tucker, Budd A.
Format: Artigo
Jezik:Inglês
Izdano: 2017
Teme:
Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5609845/
https://ncbi.nlm.nih.gov/pubmed/28806854
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpsc.30
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