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Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM

Imaging heterogeneous cellular structures using single molecule localization microscopy has been hindered by inadequate localization precision and multiplexing ability. Using fluorescent nano-diamond fiducial markers, we describe the drift correction and alignment procedures required to obtain high...

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Bibliografiska uppgifter
I publikationen:J Vis Exp
Huvudupphovsmän: Yi, Jason, Manna, Asit, Barr, Valarie A., Hong, Jennifer, Neuman, Keir C., Samelson, Lawrence E.
Materialtyp: Artigo
Språk:Inglês
Publicerad: MyJove Corporation 2017
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC5608507/
https://ncbi.nlm.nih.gov/pubmed/28671659
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55997
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