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Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM
Imaging heterogeneous cellular structures using single molecule localization microscopy has been hindered by inadequate localization precision and multiplexing ability. Using fluorescent nano-diamond fiducial markers, we describe the drift correction and alignment procedures required to obtain high...
Gardado en:
| Publicado en: | J Vis Exp |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
MyJove Corporation
2017
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5608507/ https://ncbi.nlm.nih.gov/pubmed/28671659 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55997 |
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