Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

The DNA damage response orchestrates the repair of DNA lesions that occur spontaneously, are caused by genotoxic stress, or appear in the context of programmed DNA breaks in lymphocytes. The Ataxia-Telangiectasia Mutated kinase (ATM), ATM- and Rad3-Related kinase (ATR) and the catalytic subunit of D...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Bahjat, Mahnoush, Bloedjes, Timon A., van der Veen, Amélie, de Wilde, Guus, Maas, Chiel, Guikema, Jeroen E. J.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2017
Assuntos:
Biochemistry
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5608333/
https://ncbi.nlm.nih.gov/pubmed/28654064
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55703
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