From Gene to Function: Cell-Free Electrophysiological and Optical Analysis of Ion Pumps in Nanodiscs

Nanodiscs that hold a lipid bilayer surrounded by a boundary of scaffold proteins have emerged as a powerful tool for membrane protein solubilization and analysis. By combining nanodiscs and cell-free expression technologies, even completely detergent-free membrane protein characterization protocols...

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Detalhes bibliográficos
Publicado no:Biophys J
Main Authors: Henrich, Erik, Sörmann, Janina, Eberhardt, Peter, Peetz, Oliver, Mezhyrova, Julija, Morgner, Nina, Fendler, Klaus, Dötsch, Volker, Wachtveitl, Josef, Bernhard, Frank, Bamann, Christian
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2017
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Articles
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5607034/
https://ncbi.nlm.nih.gov/pubmed/28450130
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2017.03.026
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