Automatic and adaptive heterogeneous refractive index compensation for light-sheet microscopy

Optical tissue clearing has revolutionized researchers’ ability to perform fluorescent measurements of molecules, cells, and structures within intact tissue. One common complication to all optically cleared tissue is a spatially heterogeneous refractive index, leading to light scattering and first-o...

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Veröffentlicht in:Nat Commun
Hauptverfasser: Ryan, Duncan P., Gould, Elizabeth A., Seedorf, Gregory J., Masihzadeh, Omid, Abman, Steven H., Vijayaraghavan, Sukumar, Macklin, Wendy B., Restrepo, Diego, Shepherd, Douglas P.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Publishing Group UK 2017
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5606987/
https://ncbi.nlm.nih.gov/pubmed/28931809
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-017-00514-7
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