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A Microfluidic Oxygen Gradient Demonstrates Differential Activation of the Hypoxia-Regulated Transcription Factors HIF-1α and HIF-2α
Gas-perfused microchannels generated a linear oxygen gradient via diffusion across a 100 μm polydimethylsiloxane (PDMS) membrane. The device enabled exposure of a single monolayer of cells sharing culture media to a heterogeneous oxygen landscape, thus reflecting the oxygen gradients found at the mi...
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| Publicado no: | Integr Biol (Camb) |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5603417/ https://ncbi.nlm.nih.gov/pubmed/28840922 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c7ib00099e |
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