An automated workflow for quantifying RNA transcripts in individual cells in large data-sets

Advanced molecular probing techniques such as single molecule fluorescence in situ hybridization (smFISH) or RNAscope can be used to assess the quantity and spatial location of mRNA transcripts within cells. Quantifying mRNA expression in large image sets usually involves automated counting of fluor...

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Dades bibliogràfiques
Publicat a:MethodsX
Autors principals: Pharris, Matthew C., Wu, Tzu-Ching, Chen, Xinping, Wang, Xu, Umulis, David M., Weake, Vikki M., Kinzer-Ursem, Tamara L.
Format: Artigo
Idioma:Inglês
Publicat: Elsevier 2017
Matèries:
Biochemistry, Genetics and Molecular Biology
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5596354/
https://ncbi.nlm.nih.gov/pubmed/28932696
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2017.08.002
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