An automated workflow for quantifying RNA transcripts in individual cells in large data-sets

Advanced molecular probing techniques such as single molecule fluorescence in situ hybridization (smFISH) or RNAscope can be used to assess the quantity and spatial location of mRNA transcripts within cells. Quantifying mRNA expression in large image sets usually involves automated counting of fluor...

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Detalhes bibliográficos
Publicado no:MethodsX
Main Authors: Pharris, Matthew C., Wu, Tzu-Ching, Chen, Xinping, Wang, Xu, Umulis, David M., Weake, Vikki M., Kinzer-Ursem, Tamara L.
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2017
Assuntos:
Biochemistry, Genetics and Molecular Biology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5596354/
https://ncbi.nlm.nih.gov/pubmed/28932696
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2017.08.002
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