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An automated workflow for quantifying RNA transcripts in individual cells in large data-sets

Advanced molecular probing techniques such as single molecule fluorescence in situ hybridization (smFISH) or RNAscope can be used to assess the quantity and spatial location of mRNA transcripts within cells. Quantifying mRNA expression in large image sets usually involves automated counting of fluor...

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Библиографические подробности
Опубликовано в: :MethodsX
Главные авторы: Pharris, Matthew C., Wu, Tzu-Ching, Chen, Xinping, Wang, Xu, Umulis, David M., Weake, Vikki M., Kinzer-Ursem, Tamara L.
Формат: Artigo
Язык:Inglês
Опубликовано: Elsevier 2017
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Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC5596354/
https://ncbi.nlm.nih.gov/pubmed/28932696
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2017.08.002
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