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Recycling of a selectable marker with a self-excisable plasmid in Pichia pastoris
Pichia pastoris is a widely used heterologous protein production workhorse. However, with its multiple genetic modifications to solve bottlenecks for heterologous protein productivity, P. pastoris lacks selectable markers. Existing selectable marker recycling plasmids have drawbacks (e.g., slow grow...
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| Publicado no: | Sci Rep |
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| Main Authors: | , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group UK
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5593967/ https://ncbi.nlm.nih.gov/pubmed/28894268 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-11494-5 |
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