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Recycling of a selectable marker with a self-excisable plasmid in Pichia pastoris

Pichia pastoris is a widely used heterologous protein production workhorse. However, with its multiple genetic modifications to solve bottlenecks for heterologous protein productivity, P. pastoris lacks selectable markers. Existing selectable marker recycling plasmids have drawbacks (e.g., slow grow...

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Foilsithe in:Sci Rep
Main Authors: Li, Cheng, Lin, Ying, Zheng, Xueyun, Yuan, Qingyan, Pang, Nuo, Liao, Xihao, Huang, Yuanyuan, Zhang, Xinying, Liang, Shuli
Formáid: Artigo
Teanga:Inglês
Foilsithe: Nature Publishing Group UK 2017
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5593967/
https://ncbi.nlm.nih.gov/pubmed/28894268
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-11494-5
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