A genomically modified Escherichia coli strain carrying an orthogonal E. coli histidyl-tRNA synthetase tRNA(His) pair

BACKGROUND: Development of new aminoacyl-tRNA synthetase (aaRS)•tRNA pairs is central for incorporation of novel non-canonical amino acids (ncAAs) into proteins via genetic code expansion (GCE). The Escherichia coli and Caulobacter crescentus histidyl-tRNA synthetases (HisRS) evolved divergent mecha...

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Bibliographic Details
Published in:Biochim Biophys Acta
Main Authors: Englert, Markus, Vargas-Rodriguez, Oscar, Reynolds, Noah M., Wang, Yane-Shih, Söll, Dieter, Umehara, Takuya
Format: Artigo
Language:Inglês
Published: 2017
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5592127/
https://ncbi.nlm.nih.gov/pubmed/28288813
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbagen.2017.03.003
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