Effect of pre-analytic variables on the reproducibility of qPCR relative telomere length measurement

Telomeres, long nucleotide repeats and a protein complex at chromosome ends, shorten with each cell division and are susceptible to oxidative damage. Quantitative PCR (qPCR) is a widely-used technique to measure relative telomere length (RTL) in DNA samples but is challenging to optimize and signifi...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Dagnall, Casey L., Hicks, Belynda, Teshome, Kedest, Hutchinson, Amy A., Gadalla, Shahinaz M., Khincha, Payal P., Yeager, Meredith, Savage, Sharon A.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2017
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5590866/
https://ncbi.nlm.nih.gov/pubmed/28886139
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0184098
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