EC-tagging allows cell type-specific RNA analysis

Purification of cell type-specific RNAs remains a significant challenge. One solution involves biosynthetic tagging of target RNAs. RNA tagging via incorporation of 4-thiouracil (TU) in cells expressing transgenic uracil phosphoribosyltransferase (UPRT), a method known as TU-tagging, has been used i...

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Detalles Bibliográficos
Publicado en:Nucleic Acids Res
Main Authors: Hida, Naoki, Aboukilila, Mohamed Y., Burow, Dana A., Paul, Rakesh, Greenberg, Marc M., Fazio, Michael, Beasley, Samantha, Spitale, Robert C., Cleary, Michael D.
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 2017
Assuntos:
Methods Online
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5587779/
https://ncbi.nlm.nih.gov/pubmed/28641402
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkx551
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