Insertion of the voltage-sensitive domain into circularly permuted red fluorescent protein as a design for genetically encoded voltage sensor

Visualization of electrical activity in living cells represents an important challenge in context of basic neurophysiological studies. Here we report a new voltage sensitive fluorescent indicator which response could be detected by fluorescence monitoring in a single red channel. To the best of our...

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Udgivet i:PLoS One
Main Authors: Kost, Liubov A., Nikitin, Evgeny S., Ivanova, Violetta O., Sung, Uhna, Putintseva, Ekaterina V., Chudakov, Dmitry M., Balaban, Pavel M., Lukyanov, Konstantin A., Bogdanov, Alexey M.
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2017
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5580962/
https://ncbi.nlm.nih.gov/pubmed/28863184
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0184225
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