A direct analysis of transcribed minichromosomes: all transcribed SV40 minichromosomes have a nuclease-hypersensitive region within a nucleosome-free domain.
A direct quantitative method was developed for detecting a small fraction of minichromosomes active in transcription and for analyzing their chromatin structure. For this, SV40 minichromosomes were incubated in vitro in the presence of [alpha-32P]UTP to allow elongation of the in vivo pre-initiated...
Enregistré dans:
| Publié dans: | EMBO J |
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| Auteurs principaux: | , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Nature Publishing Group
1984
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC557791/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6098456/ https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1984.tb02234.x |
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