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A direct analysis of transcribed minichromosomes: all transcribed SV40 minichromosomes have a nuclease-hypersensitive region within a nucleosome-free domain.
A direct quantitative method was developed for detecting a small fraction of minichromosomes active in transcription and for analyzing their chromatin structure. For this, SV40 minichromosomes were incubated in vitro in the presence of [alpha-32P]UTP to allow elongation of the in vivo pre-initiated...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1984
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC557791/ https://ncbi.nlm.nih.gov/pubmed/6098456 |
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